2EH7

Crystal structure of humanized KR127 FAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2001-01-09
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	100.120, 61.110, 77.530
Unit cell angles	90.00, 91.49, 90.00

Refinement procedure

Resolution	31.310 - 2.500
R-factor	0.207
Rwork	0.207
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aj7
RMSD bond length	0.006
RMSD bond angle	1.320
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.310	2.660
High resolution limit [Å]	2.500	2.500
Rmerge	0.078
Number of reflections	15758
<I/σ(I)>	5.9
Completeness [%]	96.3	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	17% PEG 4000, 10mM Hepes-NaOH, 0.2M ammonium sulfate, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 288K