2EH1

Stage V Sporolation Protein S (SpoVS) from Thermus thermophilus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-02
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97896, 0.97926, 0.9000
Spacegroup name	P 31 2 1
Unit cell lengths	71.778, 71.778, 80.833
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.900 - 2.250
R-factor	0.199
Rwork	0.199
R-free	0.22300
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.554
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM (5.0)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.080	0.219
Number of reflections	11861
<I/σ(I)>	19.8	12.8
Completeness [%]	99.9	100
Redundancy	10.4	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	100mM Imidazole, 25% propylene glycol, 200mM Zinc Acetate, 10% glycerol, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	100mM Imidazole, 25% propylene glycol, 200mM Zinc Acetate, 10% glycerol, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	100mM Imidazole, 25% propylene glycol, 200mM Zinc Acetate, 10% glycerol, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K