2EGY
Crystal structure of LysN, alpha-aminoadipate aminotransferase (substrate free form), from Thermus thermophilus HB27
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-17 |
Detector | ADSC QUAMTUM 4r |
Wavelength(s) | 0.978 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.415, 138.510, 111.745 |
Unit cell angles | 90.00, 100.21, 90.00 |
Refinement procedure
Resolution | 43.970 - 2.670 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2DTV |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.670 |
High resolution limit [Å] | 2.670 | 2.670 |
Rmerge | 0.062 | 0.232 |
Number of reflections | 48044 | |
<I/σ(I)> | 4.3 | |
Redundancy | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 17% PEG 4000, 0.1M tri-Sodium Citrate (pH6.6), 0.2M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |