2EGW
Crystal structure of rRNA methyltransferase with SAH ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2006-10-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.637, 38.774, 115.286 |
| Unit cell angles | 90.00, 115.73, 90.00 |
Refinement procedure
| Resolution | 39.350 - 2.800 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 11338 | |
| <I/σ(I)> | 23.9231 | 7.34286 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 3.84927 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |
