2EGU
Crystal structure of O-acetylserine sulfhydrase from Geobacillus kaustophilus HTA426
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-12-16 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.250, 72.250, 127.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.810 - 1.900 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1Z7W.pdb |
RMSD bond length | 0.006 |
RMSD bond angle | 1.353 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.107 | 0.529 |
Number of reflections | 27236 | |
<I/σ(I)> | 36.86 | 5.22 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.3 | 14.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | 100mM MES-NaOH, 50mM MgCl2, 27.5% PEG 4000, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |