2EGT
Crystal Structure of Hypothetical protein (AQ1549) from Aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-26 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 61.675, 61.675, 135.245 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.920 - 2.000 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e8c |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.422 |
| Number of reflections | 10922 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.7 | 20.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 0.1M CHES, 40% MPD, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
