2EGO
Crystal Structure of Tamalin PDZ Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.282, 30.034, 80.111 |
| Unit cell angles | 90.00, 132.33, 90.00 |
Refinement procedure
| Resolution | 41.140 - 1.800 |
| Rwork | 0.235 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9o |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.091 | 0.276 |
| Number of reflections | 18300 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.3 | 97.1 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 20% PEG 3350, 0.2 M potassium fluoride, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
