2EG9
Crystal structure of the truncated extracellular domain of mouse CD38
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-19 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 59.072, 176.815, 44.573 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.120 - 2.800 |
| R-factor | 0.263 |
| Rwork | 0.263 |
| R-free | 0.34000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2DF1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 11912 | |
| <I/σ(I)> | 11.614 | 5.21615 |
| Completeness [%] | 97.9 | 95.5 |
| Redundancy | 4.85275 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG 1500, 0.2M NDSB-256, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
