2EG6
The crystal structure of the ligand-free dihydroorotase from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.426, 79.674, 180.987 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.000 - 1.700 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.21400 |
| Structure solution method | Refinement |
| Starting model (for MR) | 1xge |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.417 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 1.744 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.033 | 0.327 |
| Number of reflections | 79401 | |
| <I/σ(I)> | 28.7 | |
| Completeness [%] | 96.1 | 87.5 |
| Redundancy | 3.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20-25% PEG 3350, 0.1M Na HEPES, 0.2M NaF, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
