2EFW
Crystal structure of the RTP:nRB complex from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.90 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 124.012, 129.626, 125.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.29303 |
| Rwork | 0.291 |
| R-free | 0.33221 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1F4K (with residues 72 to 88 and the DNA removed) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.532 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.091 | 0.341 |
| Number of reflections | 32161 | |
| <I/σ(I)> | 12.7 | 2.3 |
| Completeness [%] | 94.3 | 84.8 |
| Redundancy | 4.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 2% PEG 4000, 125mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | sodium acetate | ||
| 3 | 1 | 1 | HOH | ||
| 4 | 1 | 2 | PEG 4000 | ||
| 5 | 1 | 2 | sodium acetate | ||
| 6 | 1 | 2 | HOH |
