2EFQ
Crystal Structure of Thr134 to Ala of ST1022-Glutamine Complex from Sulfolobus tokodaii 7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2006-12-15 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.9794 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 103.946, 103.946, 75.039 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.760 - 2.300 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e7w |
RMSD bond length | 0.064 |
RMSD bond angle | 4.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 2.150 | 2.000 |
Rmerge | 0.103 | 0.475 |
Number of reflections | 11453 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 21.8 | 22.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30% PolypropyleneGlycol, 0.2M Sodium Citrate 0.1M Sodium cacodylate , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |