2EFP
Crystal Structure of Tyr77 to Ala of ST1022-Glutamine Complex from Sulolobus tokodaii 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 180 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-08 |
| Detector | RIGAKU RAXIS VII |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 103.687, 103.687, 74.942 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.200 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e7w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.455 |
| Number of reflections | 10689 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 9.1 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 20% PolypropyleneGlycol, 30% Isopropanol, 0.07M Sodium Citrate, 10mM Taurine, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
