2EFN
Crystal Structure of Ser 32 to Ala of ST1022 from Sulfolobus tokodaii 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2006-12-15 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 103.662, 103.662, 73.146 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 1.980 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e7w |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.099 | 0.465 |
| Number of reflections | 14388 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.7 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 20%iso PolypropyleneGlycol, 30% isopropanol 0.07M Sodium citrate, 10mM FeCl3-additive, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
