2EFH
Ara7-GDP/AtVps9a(D185N)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 |
Unit cell lengths | 58.466, 58.606, 68.974 |
Unit cell angles | 81.90, 87.42, 72.75 |
Refinement procedure
Resolution | 68.360 - 2.100 |
R-factor | 0.21599 |
Rwork | 0.214 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | nucloetide-free Ara7/AtVps9a which was solved by MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.205 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.051 | 0.277 |
Number of reflections | 47121 | |
<I/σ(I)> | 12.5 | 1.9 |
Completeness [%] | 93.2 | 70.9 |
Redundancy | 1.9 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% PEG3350, 200mM lithium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |