2EFG
TRANSLATIONAL ELONGATION FACTOR G COMPLEXED WITH GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-10-29 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.269, 106.224, 115.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.000 - 2.600 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27900 |
| Structure solution method | MIRAS |
| Starting model (for MR) | 1efg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 29278 | |
| <I/σ(I)> | 21 | 1.95 |
| Completeness [%] | 97.7 | 97.5 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 18% W/V PEG8000, 20MM TRIS-HCL, 20MM AMMONIUM ACETATE, 2MM DTT, 1MM SODIUM AZIDE, 1MM GDP, pH 7.8, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 16-20 (%(w/v)) | |
| 2 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 4 | 1 | reservoir | GDP | 1 (mM) | |
| 5 | 1 | reservoir | 1 (mM) |
