2EFE
Ara7-GDPNH2/AtVps9a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-03 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 |
Unit cell lengths | 58.235, 58.296, 69.243 |
Unit cell angles | 81.75, 86.83, 73.79 |
Refinement procedure
Resolution | 68.520 - 2.080 |
R-factor | 0.22977 |
Rwork | 0.227 |
R-free | 0.28178 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | nucleotide-free Ara7/AtVps9a which was solved by MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.458 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.180 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.064 | 0.323 |
Number of reflections | 49631 | |
<I/σ(I)> | 11.3 | 2.4 |
Completeness [%] | 96.9 | 93.5 |
Redundancy | 2.2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% PEG3350, 200mM lithium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |