2EFB
Crystal structure of the complex of goat lactoperoxidase with phosphate at 2.94 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.199, 75.587, 83.808 |
| Unit cell angles | 79.93, 77.86, 72.50 |
Refinement procedure
| Resolution | 20.000 - 2.940 |
| R-factor | 0.18319 |
| Rwork | 0.180 |
| R-free | 0.21871 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2GJ1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.485 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.990 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Number of reflections | 27189 | |
| <I/σ(I)> | 4 | 2 |
| Completeness [%] | 96.0 | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1M AMMONIUM DIHYDROGEN PHOSPHATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
