2EF9
Structural and mechanistic changes along an engineered path from metallo to non-metallo KDO8P synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 84.574, 84.574, 159.742 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.160 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fwn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.160 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 44624 |
| Completeness [%] | 1.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
