2EF8
Crystal structure of C.EcoT38IS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-13 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.420, 56.952, 61.742 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.920 - 1.950 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.25700 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.100 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.038 | 0.152 |
| Number of reflections | 13953 | |
| <I/σ(I)> | 23.1 | 9.5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.7 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 2.0M Ammonium Sulfate, 0.1M Sodium Acetate trihydrate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
