2EF7
Crystal structure of ST2348, a hypothetical protein with CBS domains from Sulfolobus tokodaii strain7
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-17 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97897, 0.9, 0.97929 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 51.420, 135.272, 41.234 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.440 - 2.100 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.26000 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.063 | 0.251 |
Number of reflections | 17492 | |
<I/σ(I)> | 32.5 | 3.89 |
Completeness [%] | 99.6 | 97.3 |
Redundancy | 6.7 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | 160mM ammonium chloride, 16%(w/v) polyethylene glycol 3350, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |