2EF6
Canavalia gladiata lectin complexed with Man1-3Man-OMe
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-01 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.43 |
Spacegroup name | P 32 |
Unit cell lengths | 69.391, 69.391, 161.298 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.920 - 2.100 |
R-factor | 0.19224 |
Rwork | 0.189 |
R-free | 0.24526 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wuv |
RMSD bond length | 0.021 |
RMSD bond angle | 2.267 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.290 | |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.040 | 0.452 |
Number of reflections | 48437 | |
<I/σ(I)> | 10.7 | 2 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | Sodium Formate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |