2EEZ
Crystal structure of alanine dehydrogenase from themus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2006-10-08 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 133.940, 133.240, 147.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.950 - 2.710 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1say |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CCP4 ((MOLREP)) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.096 | 0.361 |
Number of reflections | 71336 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.3 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 1M 1,6 hexanediol, 0.1M Na acetate, 5mM Cobalt chloride, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |