2EEY
Structure of GK0241 protein from Geobacillus kaustophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-04 |
| Detector | RIGAKU |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 105.240, 105.240, 105.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.210 - 1.940 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ekr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.078 | 0.424 |
| Number of reflections | 15314 | |
| <I/σ(I)> | 10.9 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 40 | 39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 295 | 0.12M Citrate-HCl, 0.25M Na formate, 0.1M NH(2)SO(4) and 22% PEG 4K, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
