2EEW
Guanine Riboswitch U47C mutant bound to hypoxanthine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-07-07 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 133.107, 35.258, 42.010 |
Unit cell angles | 90.00, 90.83, 90.00 |
Refinement procedure
Resolution | 14.850 - 2.250 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1U8D |
RMSD bond length | 0.004 |
RMSD bond angle | 0.800 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.850 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 13088 | |
<I/σ(I)> | 10.1 | 3.3 |
Completeness [%] | 97.6 | 99.6 |
Redundancy | 2.68 | 2.57 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% Polyethylene glycol 3000, 720mM ammonium acetate, 10mM cobalt hexammine chloride, 10mM K(+)-Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Polyethylene glycol 3000 | ||
10 | 1 | 2 | H2O | ||
2 | 1 | 1 | ammonium acetate | ||
3 | 1 | 1 | cobalt hexammine chloride | ||
4 | 1 | 1 | K(+)-Hepes | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | Polyethylene glycol 3000 | ||
7 | 1 | 2 | ammonium acetate | ||
8 | 1 | 2 | cobalt hexammine chloride | ||
9 | 1 | 2 | K(+)-Hepes |