2EES
Guanine riboswitch A21U, U75A mutant bound to hypoxanthine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-06-22 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.540, 35.960, 41.470 |
Unit cell angles | 90.00, 91.31, 90.00 |
Refinement procedure
Resolution | 19.910 - 1.750 |
Rwork | 0.207 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1U8D |
RMSD bond length | 0.021 |
RMSD bond angle | 2.100 |
Data reduction software | d*TREK |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 19129 | |
<I/σ(I)> | 15.9 | 4.1 |
Completeness [%] | 98.3 | 85.2 |
Redundancy | 3.42 | 2.81 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% Polyethylene glycol 3000, 640mM ammonium acetate, 10mM cobalt hexammine chloride, 10mM K(+)-HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Polyethylene glycol 3000 | ||
10 | 1 | 2 | H2O | ||
2 | 1 | 1 | ammonium acetate | ||
3 | 1 | 1 | cobalt hexammine chloride | ||
4 | 1 | 1 | K(+)-HEPES | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | Polyethylene glycol 3000 | ||
7 | 1 | 2 | ammonium acetate | ||
8 | 1 | 2 | cobalt hexammine chloride | ||
9 | 1 | 2 | K(+)-HEPES |