2EEQ
Mutant Y29M structure of PH0725 from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-09-22 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 104.697, 104.697, 136.615 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.900 - 2.500 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wng |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.096 | 0.438 |
Number of reflections | 26782 | |
<I/σ(I)> | 9.2 | 4.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.8 | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbach | 6.5 | 295 | 1.8M Ammonium Sulfate, 0.1M MES, 0.01M Co Chloride, 1mM DTT, pH 6.5, Microbach, temperature 295K |