2EDM

Crystal Structure of Envelope Protein VP26 from White Spot Syndrome Virus (WSSV)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9782, 0.9787, 0.9600
Spacegroup name	H 3 2
Unit cell lengths	73.915, 73.915, 199.313
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.300 - 2.200
R-factor	0.217
Rwork	0.217
R-free	0.27800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.220
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	10798
Redundancy	23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	0.1M citric acid, 3.0M sodium chloride, 1% w/v polyethylene glycol 3350, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K