2ED6

Crystal Structure of Envelope Protein VP28 from White Spot Syndrome Virus (WSSV)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9796, 0.9799, 0.9600
Spacegroup name	P 21 21 21
Unit cell lengths	105.330, 106.710, 200.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.000
R-factor	0.248
Rwork	0.248
R-free	0.28100
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.240
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.080
Number of reflections	152547
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25% PEG 8000, 0.2M Ca acetate, 0.1M Na HEPES, 1.5% 1,2,3-heptanetriol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K