2ED6
Crystal Structure of Envelope Protein VP28 from White Spot Syndrome Virus (WSSV)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9796, 0.9799, 0.9600 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.330, 106.710, 200.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.000 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.28100 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.240 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | |
Number of reflections | 152547 | |
Redundancy | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25% PEG 8000, 0.2M Ca acetate, 0.1M Na HEPES, 1.5% 1,2,3-heptanetriol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |