2ECP

THE CRYSTAL STRUCTURE OF THE E. COLI MALTODEXTRIN PHOSPHORYLASE COMPLEX

Experimental procedure

Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 180 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	76.490, 105.840, 217.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.950
R-factor	0.241 *
Rwork	0.241
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009 *
RMSD bond angle	2.100 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.600	3.110
High resolution limit [Å]	2.950	2.950
Rmerge	0.068	0.157
Number of reflections	33657
<I/σ(I)>	12.5	5.3
Completeness [%]	88.9	78.2
Redundancy	2.6	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	18 *	pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	acarbose	30 (mM)
3	1	reservoir	PEG3350	25-28 (%(w/v))
4	1	reservoir		0.1-0.6 (M)
5	1	reservoir	Tris-HCl	0.1 (M)