2EC6
Placopecten Striated Muscle Myosin II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.273, 50.366, 156.774 |
| Unit cell angles | 90.00, 101.04, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.250 |
| R-factor | 0.279 |
| Rwork | 0.279 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.073 | 0.458 |
| Number of reflections | 18825 | |
| <I/σ(I)> | 12.4 | 2 |
| Completeness [%] | 94.7 | 87.8 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 90mM CaCl2, 8.5% PEG 6K, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
