2EC2
Crystal structure of transposase from Sulfolobus tokodaii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-10 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.118, 66.566, 69.472 |
| Unit cell angles | 107.96, 104.39, 110.72 |
Refinement procedure
| Resolution | 25.780 - 2.800 |
| R-factor | 0.20629 |
| Rwork | 0.201 |
| R-free | 0.29861 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f5g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.385 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.057 | 0.230 |
| Number of reflections | 18474 | |
| <I/σ(I)> | 28.1 | |
| Completeness [%] | 93.0 | 82 |
| Redundancy | 2.9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 20% PEG 4000, 0.2M Lithium Sulfate, 0.1M Sodium Acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
