2EBY

Crystal structure of a hypothetical protein from E. Coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	180
Detector technology	CCD
Collection date	2006-12-18
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.97895, 0.97943, 0.9000
Spacegroup name	P 32 2 1
Unit cell lengths	89.793, 89.793, 54.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.930 - 2.250
R-factor	0.228
Rwork	0.228
R-free	0.24800
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.077	0.306
Number of reflections	12218
Completeness [%]	100.0	100
Redundancy	10.2	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	9.4	293	1.93M Ammonium sulfate, 0.1M CHES, pH 9.4, LIQUID DIFFUSION, temperature 293K