2EBO
CORE STRUCTURE OF GP2 FROM EBOLA VIRUS
Experimental procedure
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-12 |
Detector | ADSC |
Spacegroup name | P 62 |
Unit cell lengths | 75.660, 75.660, 67.930 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.26900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mof |
RMSD bond length | 0.009 |
RMSD bond angle | 19.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (0.5) |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 1.920 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.037 | 0.309 |
Total number of observations | 86630 * | |
Number of reflections | 17326 | |
<I/σ(I)> | 18.8 | 3.5 |
Completeness [%] | 98.9 | 99.1 |
Redundancy | 4.9 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | isopropanol | 16-18 (%) | |
3 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
4 | 1 | reservoir | 100-200 (mM) |