2EBO

CORE STRUCTURE OF GP2 FROM EBOLA VIRUS

Experimental procedure

Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-12
Detector	ADSC
Spacegroup name	P 62
Unit cell lengths	75.660, 75.660, 67.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.205
Rwork	0.205
R-free	0.26900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mof
RMSD bond length	0.009
RMSD bond angle	19.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (0.5)
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	1.920
High resolution limit [Å]	1.900	1.900
Rmerge	0.037	0.309
Total number of observations	86630 *
Number of reflections	17326
<I/σ(I)>	18.8	3.5
Completeness [%]	98.9	99.1
Redundancy	4.9	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6		pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	isopropanol	16-18 (%)
3	1	reservoir	sodium acetate	100 (mM)	pH4.6
4	1	reservoir		100-200 (mM)