2EB8
Crystal Structure of Cu(II)(Sal-Phe)/apo-Myoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-29 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.691, 59.132, 74.188 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.700 - 1.650 |
| R-factor | 0.20546 |
| Rwork | 0.203 |
| R-free | 0.24636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v9q |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.483 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.092 | 0.293 |
| Number of reflections | 17781 | |
| <I/σ(I)> | 40.59 | |
| Completeness [%] | 98.8 | 96.4 |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 1.3M Sodium, Pottasium Phosphate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
