2EB7
Crystal structure of the hypothetical regulator from Sulfolobus tokodaii 7
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2006-07-18 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97896, 0.97930, 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 45.919, 45.919, 138.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.280 - 1.800 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.26800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.065 | 0.565 |
| Number of reflections | 14613 | |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 12.7 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 18% PEG 8000, 0.1M Sodium cacodylate, 0.2M Calcium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
