2EAY
Crystal Structure Of Biotin Protein Ligase From Aquifex Aeolicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-02 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.076, 60.589, 73.106 |
Unit cell angles | 90.00, 91.99, 90.00 |
Refinement procedure
Resolution | 36.530 - 1.950 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wqw |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.530 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.049 | 0.328 |
Number of reflections | 33213 | |
<I/σ(I)> | 14.7 | 2.1 |
Completeness [%] | 94.2 | 96.4 |
Redundancy | 3.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.4 | 295 | 27.5w/v(%) PEG 4K, 0.1M Hepes-NaOH, pH 7.4, microbatch, temperature 295K |