2EAX
Crystal structure of human PGRP-IBETAC in complex with glycosamyl muramyl pentapeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.940, 61.390, 97.950 |
| Unit cell angles | 90.00, 96.45, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.215 |
| Rwork | 0.210 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eav |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.064 | 0.273 |
| Number of reflections | 27509 | |
| Completeness [%] | 97.9 | 96.72 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 15% (v/v) Tacsimate, 0.1M Hepes pH 7.0, 2% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
