2EAW
Human UMP Synthase (C-terminal Domain- Orotidine 5'-Monophosphate Decarboxylase)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-01 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.571, 59.370, 69.863 |
| Unit cell angles | 90.00, 114.30, 90.00 |
Refinement procedure
| Resolution | 63.630 - 2.880 |
| R-factor | 0.26179 |
| Rwork | 0.259 |
| R-free | 0.30737 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dqw |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.993 |
| Data reduction software | SAINT (PLUS) |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.670 | 2.900 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.141 | 0.400 |
| Number of reflections | 11348 | |
| <I/σ(I)> | 4.29 | 2.04 |
| Completeness [%] | 97.8 | 28.9 |
| Redundancy | 1.84 | 0.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 2.28M Ammonium sulfate, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
