2EAU
Crystal structure of the SR CA2+-ATPASE with bound CPA in the presence of curcumin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.8000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 71.648, 71.648, 586.246 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.2468 |
Rwork | 0.247 |
R-free | 0.27180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iwo |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.880 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.141 | 0.534 |
Number of reflections | 39493 | |
<I/σ(I)> | 32 | 7.2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 17.1 | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.1 | 283 | PEG 400, pH 6.1, MICRODIALYSIS, temperature 283K |