2EAT
Crystal structure of the SR CA2+-ATPASE with bound CPA and TG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.8000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.899, 95.645, 155.100 |
Unit cell angles | 90.00, 95.24, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.900 |
R-factor | 0.2465 |
Rwork | 0.244 |
R-free | 0.29223 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iwo |
RMSD bond length | 0.011 |
RMSD bond angle | 1.416 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.970 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.048 | 0.361 |
Number of reflections | 40344 | |
<I/σ(I)> | 33.6 | 2 |
Completeness [%] | 99.2 | 92.5 |
Redundancy | 7 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.1 | 283 | PEG 400, pH 6.1, MICRODIALYSIS, temperature 283K |