2EAE
Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complexes with products
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.519, 70.878, 170.979 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.630 - 1.800 |
| R-factor | 0.18186 |
| Rwork | 0.180 |
| R-free | 0.21886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.460 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 171.500 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 69085 | |
| Completeness [%] | 96.8 | 96.7 |
| Redundancy | 12.1 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES (pH6.0), 15% PEGMME2000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
