2EAC
Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.668, 111.702, 98.346 |
| Unit cell angles | 90.00, 95.31, 90.00 |
Refinement procedure
| Resolution | 48.970 - 2.100 |
| R-factor | 0.15264 |
| Rwork | 0.150 |
| R-free | 0.19757 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.505 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.060 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 113799 | |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.2 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1M Tris (pH7.0), 10% iso-propanol, 10% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
