2EA9
Crystal structure of a hypothetical protein JW2626 from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-12 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 32 |
| Unit cell lengths | 49.865, 49.865, 38.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.760 - 2.100 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2H28 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (1.3.3) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.075 | 0.325 |
| Number of reflections | 6091 | |
| <I/σ(I)> | 12.5 | 3.4 |
| Completeness [%] | 97.3 | 81.4 |
| Redundancy | 5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1M HEPES, 1.0M Succinic acid, 1.0% PEG MME 2000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
