2EA7
Crystal Structure of Adzuki Bean 7S Globulin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-30 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.598, 48.550, 119.765 |
| Unit cell angles | 90.00, 97.12, 90.00 |
Refinement procedure
| Resolution | 14.980 - 1.800 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ipk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.054 | 0.338 |
| Number of reflections | 104746 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 97.8 | 92.9 |
| Redundancy | 4.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 34% MPD, 0.1M acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
