2E9Q
Recombinant pro-11S globulin of pumpkin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-11 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 1.0 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 188.997, 188.997, 188.997 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.940 - 2.200 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.26700 |
| Structure solution method | MIR |
| Starting model (for MR) | 2evx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.045 | 0.442 |
| Number of reflections | 28317 | |
| <I/σ(I)> | 122 | 5.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2M NaCl, 0.1M Na/K phosphate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
