2E8Y
Crystal structure of pullulanase type I from Bacillus subtilis str. 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.8000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.562, 127.682, 189.251 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.110 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fgz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.300 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.078 | 0.314 |
| Number of reflections | 99003 | |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 10% PEG 4000, 0.1M sodium acetate buffer, 0.2M magnesium acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
