2E8Q
Structural study of Project ID PH0725 from Pyrococcus horikoshii OT3 (K19M)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-10-25 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 104.693, 104.693, 137.942 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.870 - 2.500 |
R-factor | 0.211 |
Rwork | 0.210 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.058 | 0.403 |
Number of reflections | 27146 | |
<I/σ(I)> | 18.9 | 6.83 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.3 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.5 | 291 | 3.85M Na Form, 0.1M Acet., pH 5.5, MICROBATCH, temperature 291K |