2E8G

The structure of protein from P. horikoshii at 1.7 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-26
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	49.186, 71.691, 69.193
Unit cell angles	90.00, 96.15, 90.00

Refinement procedure

Resolution	34.400 - 1.700
R-factor	0.203
Rwork	0.199
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.437
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.401	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.049	0.353
Number of reflections	52434
<I/σ(I)>	19.7	2.9
Completeness [%]	99.7	99.3
Redundancy	4.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	298	18% PEG 6000, 5% Glycerol, 0.1M MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.8	298	18% PEG 6000, 5% Glycerol, 0.1M MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K