2E8B
Crystal structure of the putative protein (Aq1419) from Aquifex aeolicus VF5
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97891, 0.97944, 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.106, 56.795, 62.716 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.620 - 1.610 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.26100 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.100 | 1.670 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.058 | 0.341 |
| Number of reflections | 24264 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.82 | 6.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 0.2M Ammonium Iodide, 20% PEG3350, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
