2E87
Crystal structure of hypothetical GTP-binding protein PH1320 from Pyrococcus horikoshii OT3, in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-04 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.978997 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.253, 99.191, 126.135 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.600 - 2.350 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.25500 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 25473 | |
<I/σ(I)> | 15.8132 | 2.79832 |
Completeness [%] | 98.5 | 91.6 |
Redundancy | 4.33557 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | 1.5M ammonium sulfate, 0.2M potassium sodium tartrate tetrahydrate, 0.1M tri-sodium citrate dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |